Establishment of cell line stably expressing INSIG2 and its effects on fat metabolism
- VernacularTitle:INSIG2稳定表达细胞系的建立及该基因对脂肪代谢的影响
- Author:
Ke CHEN
;
Zhaohui MO
;
Xiaowei XING
;
Pingan HU
;
Yanhong XIE
- Publication Type:Journal Article
- Keywords:
INSIG2;
3T3-L1 cell;
fat metabolism
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the cell line stably expressing INSIG2 and observe its effecet on fat metabolism after overexpression of INSIG2.Methods The eukaryotic expression plasmid pcDNA3.1(+)-INSIG2 was constructed,which was transfected into 3T3-L1 cells.The expression of INSIG2 and related genes were detected by RT-PCR and immunohistochemistry,the contents of FFA in cell culture medium and adipocyte differentiation were detected by ELISA and Oil Red "O"staining respectively.Results After pcDNA3.1(+)-INSIG2 was transfected into the 3T3-L1 cells,the expression of INSIG1 mRNA and FAS mRNA were down-regulated,the content of FFA in the cell culture medium was decreased and adipocyte differentiation was drepressed.Conclusion The cell line stably expressing INSIG2 was successfully established,the transfected INSIG2 may have a drepressant effect on fat metabolism.
