In vivo quantitative measurement of glutamate & glutamine in zelanian rabbits' muscle using MRS
- VernacularTitle:MRS测定新西兰兔活体肌肉组织内谷氨酸、谷氨酰胺总浓度的方法探讨
- Author:
Fan ZHANG
;
Jianchun YU
;
Yueping FAN
;
Guizhen HE
;
Ling HU
;
Xiaozhen LI
;
Zhengyu JIN
- Publication Type:Journal Article
- Keywords:
MRS;
muscle;
glutamate;
glutamine;
quantitatively measurement
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Use MRS technology to detect in vivo Glx concentration of Zelanian rabbits' muscle.Methods Tweenty Zelanian rabbits are collected,SS-PRESS sequence is applied to check their Signal Ratio of Glx/TCr.After deta collection,draw blood and do the biopsy of the FOV's muscle tissue immediately.Mensurate the rabbits' blood Crn concentration,Glx and TCr concentration in muscle.Research the correlation between the above factors,attempt using the MRS Glx/TCr Signal Ratio and blood Crn concentration to predict muscular Glx concentration.Results The correlation between MRS Glx/TCr Peak Ratio and muscular Glx/blood Crn concentration ratio is 0.681.A linear regression formula is obtained: The predict Value of Glx concentration in muscle(?mol/g muscle)= Glx/TCr Peak Ratio ? Crn concentration in blood(mg/dl)?28.754-0.631.Conclusion Using a linear regression formula to predict the muscular Glx concentration,the results can reflect the level of the true values without biopsy,though it's not accurate enough for quantitatively analysis.
