Regulation of Notch1 activity by Rab5 in pancreatic cancer BxPC3 cells
- VernacularTitle:Rab5在胰腺癌细胞BxPC3中对Notch1活性的调节
- Author:
Juan ZHANG
;
Hefen YU
;
Zesheng WANG
;
Yuxiang ZHANG
- Publication Type:Journal Article
- Keywords:
pancreatic cancer;
BxPC3;
Notch1;
Rab5;
wortmannin;
LY294002;
endocytosis
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidates the regulation of Notch1 activity by Rab5 which controls endosome fusion.Methods Rab5 expression of BxPC3 cells were inhibited by siRNA interference,the changes of Notch ICD protein were measured by Western blot.We use Wortmannin and LY294002 which inhibit the acticity of Phosphatidylinositol(PI) 3-kinases to investigate Notch ICD protein level.Results After inhibiting Rab5 expression or the activity of Phosphatidylinositol(PI) 3-kinases,Notch ICD protein level and the growth of BxPC3 cells decreased.Conclusion In BxPC3 cells,Rab5 and Phosphatidylinositol(PI) 3-kinases are important to regulate Notch1 activity and the activation of Notch is dependent on endocytosis.
