Difference between the bone marrow and peripheral blood engraftment evidence after allogeneic hematopoietic stem cell transplantation by PCR-STR
- VernacularTitle:PCR-STR用于异基因造血干细胞移植后的骨髓和外周血嵌合状态的监测及作用
- Author:
Wenjie XIA
;
Yongshui FU
;
Xin YE
- Publication Type:Journal Article
- Keywords:
PCR-STR;
Allogeneic hematopoietic stem cell transplantation;
Chimerism
- From:
Chinese Journal of Blood Transfusion
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the difference between the bone marrow and peripheral blood engraftment evidence after allogeneic haemopoietic stem cell transplantation(Allo-HSCT) using PCR-STR. Methods DNA from peripheral blood or bone marrow of donors and recipients in different phases were extracted,and 16 STR loci with high polymorphism were amplified by PCR.Separation of the PCR products and fluorescence detection were performed by ABIprism 3100 Genetic Analyzer with capillary electrophoresis.Results The 16 patients included in the study had different levels of engraftment.Twelve patients displayed complete chimerism,while 5 patients showed mixed chimerism.One patient was keeping continuance of remission.The decrease of donor DNA amounts in mixed chimerism was earlier in bone marrow than that in peripheral blood(P
