Effect of Ceftriaxone on glutamate-induced cerebral cortical neurocyte injury
- VernacularTitle:头孢曲松钠对谷氨酸诱导脑皮质神经元损伤的影响
- Author:
Chunhua LIU
;
Weizhi WANG
- Publication Type:Journal Article
- Keywords:
Ceftriaxone;
glutamate;
intracellular calcium;
glutamate transporters
- From:
Journal of Clinical Neurology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Ceftriaxone on glutamate-induced neurocyte injury. Methods Cultured rat cerebral cortical neurocytes were divided into Ceftriaxone (A)group, glutamate(B) group ,Ceftriaxone-pretreated (C) group and control (D) group. Observe morphous and MTT colorimetry were used to detect cytoactive. Laser scanning confocal microscope was used to measure the change of Ca2+ in rest state and after brief glutamate-induced Ca2+ loading. RT-PCR was used to determine the change of GLT1 mRNA expression.Results Compared with B group, cellular morphous was closer to normal in C group. Absorptance values of groups A,B,C,D were 0.795?0.010, 0.624?0.028, 0.738?0.021 and 0.813?0.023, respectively, which there were lower in groups B and C compared with group D, but absorptance value was higher in group C than that in group B(allP
