Construction of a lentiviral expression vector that inhibits enhanced green fluorescent proteins
- VernacularTitle:抑制绿色荧光蛋白的慢病毒表达载体的构建
- Author:
Tao YUAN
;
Shaoli DENG
;
Ji XIA
;
Hengliu HUANG
- Publication Type:Journal Article
- Keywords:
Lentivirus vector;
RNAi;
Enhanced green fluorescent protein
- From:
Journal of Medical Postgraduates
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct a lentiviral expression vector that inhibits enhanced green fluorescent proteins(EGFP).Methods: The EGFP shRNA was cloned into the pLL3.7 lentivirus vector digested with XbaⅠ/XhoⅠ.J558L and Hela cells were infected by lentiviruses,and the EGFP expression was observed with the fluorescence microscope.Results: The EGFP RNAi sequence was correctly cloned into the lentivirus vector with the U6 promoter,and the EGFP expressions of Hela and J558L cells were suppressed after infected by lentivirus containing EGFP shRNA. Conclusion: The lentivirus vector effectively inhibited EGFP expression,which could be used to mediate further researches on gene function.
