WU Polyomavirus Detected by Real-time PCR
- VernacularTitle:WU多瘤病毒实时荧光聚合酶链检测方法的建立与应用
- Author:
Juanfang SONG
;
Jianming XING
- Publication Type:Journal Article
- Keywords:
WU Polyomavirus;
Real-time PCR;
Detection
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a rapid, sensitive and specific real-time PCR assay for the detection of WU polyomavirus.METHODS The VP2 gene of WU polyomavirus was selected as target gene.The specific primers and TaqMan probes according to the principle of the TaqMan real-time PCR were designed and synthesized,we optimized the PCR reaction,and detected 394 clinical samples.The sensitivity,specificity,reproducibility and dynamic range of the methods were determined.RESULTS The real-time PCR was established and applied successfully to detect the WU polyomavirus.The real-time PCR assay showed excellent linearity between the log of target input and CT value,demonstrating that the assay had a dynamic range of at least 8 logs between 1.0?100 and 1.0?107 copies;the coefficient of variation of intra-assay and inter-assay was less than 5%.Among these 394 specimens,3(0.76%) were positive for WU polyomavirus.CONCLUSIONS The real-time TaqMan PCR is a rapid,specific and sensitive method to detect WU polyomavirus.It can be used in screening large numbers of samples at the same time and establish the solid technological base for clinical diagnosis and epidemiology study.
