Purification,renaturation and antiviral effects of recombinant cyanovirin-N on herpes simplex virus type 1
- VernacularTitle:重组蓝藻抗病毒蛋白N的纯化复性及其抗单纯疱疹病毒1型活性的研究
- Author:
Zongtao LIU
;
Hong YU
;
Yanmei YIN
;
Dan LI
;
Wenqing ZHANG
- Publication Type:Journal Article
- Keywords:
cyanovirin-N;
purification;
renaturation;
herpes simplex virus type 1;
antivirus
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify,renature and explore the antiviral effects of recombinant cyanovirin-N(CV-N)on herpes simplex virus type 1(HSV-1).Methods The recombinant CV-N was purified with Ni Sepharose column and renatured by dilution method.The antiviral activities of CV-N were carried out in Vero cells by observing cytopathic effect(CPE)and by using MTT colorimetric assay for vital cell rate.Results SDS-PAGE showed that the purified protein was in the position of 11KDa with only one clear band.The renatured CV-N had little cytotoxic effect on Vero cells,it could not directly inactivate HSV-1 infectivity.CV-N not only interfered in adsorption of HSV-1 to Vero cells but also inhibited HSV-1 biosynthesis in the cells,which were more effective than the positive control Acyclovir.Conclusion CV-N exhibited pronounced antiviral activities agaist HSV-1,further development of CV-N might yield novel candidates of antiviral drugs.
