Determination of contents of aconitine in Shenhailong capsules by RP-HPLC
- VernacularTitle:RP-HPLC测定深海龙胶囊中乌头碱的含量
- Author:
Shuxia GUO
;
Yahua XIE
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Shenhailong capsules;
aconitine;
RP-HPLC
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for determination of aconitine in Shenhailong capsules.Methods Zorbax SB-C18column(4.6 mm?250 mm,5 ?m)was used,with(methanol∶water∶triethyl amine)60∶40∶0.1,as the mobile phase.The flow rate was 1.0mL?min-1and the column temperature was at 30℃.The detection wavelength was 240nm.Results Aconitine concentration presented a good linear range of 0.04?10-3～4?10-3g?L-1(r = 0.9999).with the average recovery of 98.4%,and RSD :1.0%.ConclusionThe method was accurate,nice in reproducibility and with few interference.It could be used for quality control of Shenhailong capsules.
