Chloramphenicol-resistant Escherichia coli and Its Resistance Genes Isolated Strains from Old Patients
- VernacularTitle:老年患者大肠埃希菌分离株对氯霉素耐药性及耐药相关基因研究
- Author:
Faxiang JIN
;
Huajun WANG
;
Jialiang TANG
;
Shuifa LI
;
Jianping ZHONG
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Polymerase chain reaction;
Resistance gene;
Sequence analysis
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the chloramphenicol-resistant gene in Escherichia coli strains isolated from the old patients.METHODS Twenty strains of E.coli were clinically isolated.The resistance genes were analyzed by polymerase chain reaction(PCR) and verified by DNA sequencing.RESULTS The positive rates of catB and cmlA were 20% and 20%,respectively.Two strains of E.coli were found containing catB gene and cmlA gene.CONCLUSIONS The resistance to chloramphenicol in these 20 strains of E.coli has a close relation to antimicrobial-resistance gene existed,and the genes of catB and cmlA in E.coli are all firstly found and reported in China.
