Differentiation of fetal-bone mesenchymal stem cells induced by three kinds of connective tissue in vitro
- VernacularTitle:三种结缔组织体外诱导胎儿骨髓间充质干细胞的分化
- Author:
Yan YANG
;
Zheng DUAN
;
Rong JIANG
;
Weiwei WANG
- Publication Type:Journal Article
- Keywords:
fetus;
connective tissue;
bone marrow-derived mesenchymal stem cells;
induction;
differentiation
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the inducing effect of three kinds of connective tissue from amnion,fetal mesentery and intestinal submucosa on the differentiation of fetal BMSCs in vitro.Methods The epithelium on three kinds of connective tissue were sampled and EGF and IGF-1 in these tissue clumpes were detected.DAPI-P3-BMSCs were planted on the surface of three kinds of connective tissue to culture.There were connective tissue induced groups,connective tissue and EGF and IGF-1 induced groups,EGF and IGF-1 induced as control group.The tissue clump was collected at 8 d、10 d、12 d,to compare their inducing effect on the differentiation of BMSCs.Results All of BMSCs induced by three kinds of connective tissue and growth factors expressed CK or EMA.The effect of unite-induced groups was stronger than those groups induced by only connective tissue,P
