Expression and construction of an adeno-associated virus vector containing dual genes-GDNF and TH
- VernacularTitle:AAV-GDNF/TH双基因载体的构建及表达
- Author:
Min ZHANG
;
Hui YANG
- Publication Type:Journal Article
- Keywords:
adeno-associated virus;
Parkinson's disease;
TH;
GDNF
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Rat glial cell derived neurotrophic factor(GDNF) gene and tyrosine hydroxylase(TH) gene were widely used in gene therapy of Parkinson's Disease(PD).The present study was designed to investigate whether the two target genes GDNF and TH could express simultaneously in one adeno-associated virus(AAV)vector and to discuss the probability of using it in gene therapy of PD.Methods RNA of GDNF and TH in HEK293 packaging cells were examined by reverse transcription-polymerase chain reaction(RT-PCR).Expressions of GDNF and TH in infected bone marrow stromal cells(BMSCs) and rat brain coronal sections were examined with immunocytochemistry and immunohistochemistry assay.Results RNA of GDNF and TH were detected in HEK293 packaging cells.Expressions of this two genes were observed in both bone marrow stromal cells and brain sections of rats.Theexpression efficiency of AAV-LacZ was about 50% in HEK293 packaging cells and approximately 15% in infected BMSCs and the expressions of TH and GDNF on the AAV-GDNF/TH virus injected points both had significant differences(P
