Screening of plasmids targeted to GCN5 and detection of their effects on MSCs differentiation
- VernacularTitle:GCN5特异性质粒筛选及其对MSCs分化的影响
- Author:
Li LI
;
Jing ZHU
;
Chuan FENG
;
Jie TIAN
- Publication Type:Journal Article
- Keywords:
GCN5;
RNA interference;
plasmid;
histone acetylation
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the valid plasmid targeted toGCN5 among the constructed recombinant plasmids;and to explore the effects of histone acetylizad modification in regulating MSCs differentiation.Methods Exstract the constructed plasmids and transfect them into MSCs induced by 5-aza.For 24 h,observe MSCs;detect the transfect efficiency by flow cytometry;detect expression of protein GCN5 by Western-blot;detect the HAT activity by ELISA.Results Transfect efficiency was more than 20%;Expression of protein GCN5 and HAT activity had no difference in group ZJ1 and ZJ2,but had a significant difference to that in group ZJ3.HAT activity of experiment group was significantly lower than that of control groups.Conclusion The inhibition state of histone acetylation results in plasmid ZJ3,it can inhibit the differentiation process of MSCs.The results provide data for the clinical application of MSCs transplantation.
