L-3-n-butylphthalide inhibits expression of nuclear factor-?B and I?B-? in primary cultured murine brain glial cells following oxygen-glucose deprivation/reoxygenation
- VernacularTitle:左旋丁基苯酞抑制氧糖剥夺/复氧所致原代培养小鼠脑胶质细胞内NF-?B和I?B-?蛋白表达
- Author:
Shanshan DENG
;
Yong LUO
- Publication Type:Journal Article
- Keywords:
l-3-n-butylphthalide;
l-NBP;
glial cell;
nuclear factor-?B;
I?B-?;
inflammatory reaction
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of l-3-n-butylphthalide(l-NBP)on the of nuclear factor-?B and I?B-? in primary cultured murine brain glial cells following oxygen-glucose deprivation/ reoxygenation(OGD/R).Methods Cultured mouse brain glial cells in vitro and established OGD/R model.Glial cells were divided into five groups: normal control(NC)group,OGD 24 h/R 24 h group,l-NBP 20 ?mol/L group,l-NBP 100 ?mol/L group,l-NBP 500 ?mol/L group.The protein expression of NF-?B in nuclear and I?B-? in endochylema were analyzed by Western blot.Results Comparing with NC group,OGD 24 h/R 24 h group showed a significantly higher expression of NF-?B(P
