Attenuated endocytosis by down-regulation of cortactin protein in cancer cells
- VernacularTitle:在癌细胞中下调皮质肌动蛋白使细胞内吞作用减弱
- Author:
Li CHEN
;
Jianwei ZHU
;
Xi ZHAN
- Publication Type:Journal Article
- Keywords:
cortactin;
endocytosis;
siRNA;
interference
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study cortactin function in cancer cell endocytosis.Methods We applied cortactin siRNA interference to MDA-Mb-231,a human breast adenocarcinoma cell line in which cortactin was over-expressed,and introduced anti-cortactin immunoreagents into the cells with BioPorter system to interfere with cortactin function in vivo.Capture-ELISA assay was used to measure transferrin uptake.We used immunoblot assay to assess the effect of cortactin knock-down and immunoflurescence microscopy to examine the effect of cortactin down-regulation on transferrin uptake.Results Interference of cortactin function in cells resulted in impairment of transferrin endocytosis.Transferrin fluorescent intensity in cytoplasm in cortactin siRNA treated-cells was significantly reduced in comparison to that of mock-treated cells.Less than 50% of cells subjected to cortactin siRNA treatment had normal transferrin uptake.Endocytosis in MDA-Mb-231 cells introduced with cortactin antibodies was impaired as well,showing a 30%～ 60% reduction in transferrin uptake.Conclusion Crtactin,an actin-binding protein,plays an essential role in cell endocytosis.
