Effect of small heterocyclic compound S1 on inducing PC3 cell line apoptosis
- VernacularTitle:小分子化合物S1诱导PC3细胞凋亡的作用
- Author:
Congmei WU
;
Qing YANG
;
Shunzi JIN
- Publication Type:Journal Article
- Keywords:
PC3 cell line;
apoptosis;
mechanism
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of small heterocyclic compound 8-oxo-3-thiomorpholino-8H-acenaphtho pyrrole-9-carbonitrile(S1) on apoptosis of human androgen independent prostatic carcinoma cells line(PC3) and its mechanism.Methods PC3 cells were cultivated,and divided into different groups: 10.00,5.00,1.00,0.50,0.10,0.05 and 0.01 ?mol?L-1 S1 groups,meanwhile,PC3 control group and cyclophosphamide group were set up.MTT was used to detect the inhibitory rate of PC3 cell proliferation.Flow cytometry was used to detect the inducing effect of S1 on apoptosis of PC3 cells.Caspase 3,8,9 kits were used to detect apoptosis route.Results The inhibitory rates of PC3 cells induced by 0.10-10.00 ?mol?L-1 S1 were significantly higher than that in cyclophosphamide group(P
