Analysis of enzymological characterization of N-V proteinase with fibrinolytic activity
- VernacularTitle:一种新的具有纤溶活性的N-V蛋白酶酶学性质分析
- Author:
Qi LI
;
Zhao WANG
;
Jizhou ZHANG
;
Min HONG
- Publication Type:Journal Article
- Keywords:
N-V proteinase;
Km;
serine endopetidases
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the optimum reaction temperature,pH,Km of N-V proteinase and determine its enzymological classification.Methods The fibrin plate method was used to determine the optimum reaction temperature and pH of N-V proteinase.The Km of N-V proteinase was detected by using Leu-pNA as substrate.E64,PMSF,zymofren and 1,10-phenanthroline were used as inhibitors to determine proteinase classification of N-V proteinase.Results The optimum reaction temperature was 50℃,pH was 8-9,Km was 1.97?10-3mol?L-1.PSMF inhibited the fibrinolytic activity of N-V proteinase(P0.05).Conclusion N-V proteinase belongs to serine proteinase.
