Effect of panaxadiols on AQP1 expression in lungs of hemorrhage-LPS two-hits rats
- VernacularTitle:人参二醇皂苷对失血性休克复合内毒素二次打击大鼠肺组织水通道蛋白1表达的影响
- Author:
Zhenxiang YU
;
Heng ZHAO
;
Shucheng HUA
;
Xuejian ZHAO
- Publication Type:Journal Article
- Keywords:
panaxadiols;
aquaporins;
two hits;
endotoxins shock,hemorrhagic
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alteration of aquaporin 1(AQP1) expression in lung tissues in hemorrhage-lipopolysaccharide(LPS) two-hits rats and the effects of panaxadiols(PDS)and dexamthasone(Dex) on it.Methods The rat model of acute lung injury was built with hemorrhage-LPS two hits.The experiment was divided into control group(S),two-hits model group(HL),DEX group(HLD),and PDS group(HLP).The pathological changes of lung tissue were examined by HE staining.The expression of AQP1 was analyzed by RT-PCR,Western blotting and immunohistochemical staining.Results ① Significant inflammatory changes in pulmonary interstitial of rats in HL group were observed.However,in HLD group and HLP group,the pulmonary pathologic changes were much slighter.② AQP1 mRNA and protein expressions in lung tissues in HL group were significantly decreased compared with others groups(P
