Effects of Recombinant Angiotensin-converting enzyme 2 Gene on Oxidative Stress Levels in Human Endothelial Cells
- VernacularTitle:血管紧张素转换酶2基因转染对人血管内皮细胞中氧化应激水平的影响
- Author:
Jiuchang ZHONG
- Publication Type:Journal Article
- Keywords:
Angiotensin-converting enzyme 2;
p22~(phox);
Malonyldialdehyde;
Oxidative stress;
Essential hypertension
- From:
Chinese Journal of Hypertension
2007;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Backgroup and objective Angiotensin-converting enzyme 2 (ACE2) is the first known homologue of human ACE gene up till now,however,its regulatory role in oxidative stress remains unclear.Our aim is to in- vestigate the effects of recombinant ACE2 gene transfer on the expression of p22~(phox),a key subunit of NADPH oxi- dase,and malonyldialdehyde (MDA) levels induced by angiotension(Ang) Ⅱ in cultured human endothelial cells. Methods A recombinant plasmid encompassing human ACE2 gene (pACE2) was constructed and transfected into these cells.The mRNA and protein levels of p22~(phox) in endothelial cells were determined by real-time PCR and Western blotting,respectively.MDA contents were measured by thiobarbiturie acid colorimetrie method in cells. Results The mRNA and protein expressions of p22~(phox) were drastically enhanced after exposures of endothelial cells to Ang Ⅱ(100 nmol/L) and Ang Ⅳ(100 nmol/L),accompanied by an increase in MDA contents (n=6,P
