Effect of dexamethasone on proliferation of human periodontal ligament fibroblasts cultivated in vitro
- VernacularTitle:地塞米松对体外培养人牙周膜成纤维细胞增殖的影响
- Author:
Yungang LUO
;
Xiaoqiu LIU
;
Xiaomin ZHU
;
Xiaodong YAN
- Publication Type:Journal Article
- Keywords:
human periodontal ligament fibroblasts;
culture in vitro;
fetal bovine serium;
dexamethasone
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dexamethasone on proliferation of human periodontal ligament fibroblasts(hPDLF) cultivated in vitro,and search for optimal culture condition for hPDLF in vitro,and provide basis for further study on regeneration of periodontinum.Methods The hPDLF cultivated in vitro were divided into 5 groups and cultivated in 5 different culture media separately which all included 10% fetal bovine serium: ①control group(no DEX);②5 mg?L-1DEX;③10 mg?L-1 DEX;④20 mg?L-1 DEX;⑤50 mg?L-1 DEX.The proliferation of hPDLF was assyed by MTT method.The phase contrast microscope was used to observe the morphological changes of the cells.Results The PDLF appeared aggregation and cells on bottom of culture bottle showed squamae-shape after inoculated on plate with 24 hole for 3 d.There were more layers of smaller hPDLF with blunt prominency came forth after cultivated in DEX culture media.MTT method showed that DEX promoted the proliferation of hPDLF obviously compared with control group(P
