Isolation and culturing of highly purified glandular epithelial cells from human endometrial specimens
- VernacularTitle:高纯度子宫内膜腺上皮细胞的体外分离和培养
- Author:
Lei ZHANG
;
Qinping LIAO
- Publication Type:Journal Article
- Keywords:
Endometrium;
Cell culture;
Glandular epithelial cell
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an improved method for the primary isolation and culture of highly purified endometrial glandular epithelial cells in vitro.Methods: Normal endometrial tissues were isolated and cultured in vitro by double collagenase digestion and selective wall-pasting.Results: Highly purified endometrial epithelial cells were successfully isolated and cultured.Conclusion: Epithelial and interstitial cells of the endometrium can be successfully isolated with high purity by double collagenase digestion and selective wall-pasting.
