Isolation and Characterization of Bacteriophage for Klebsiella pneumoniae
- VernacularTitle:肺炎克雷伯菌噬菌体的分离和生物学特性
- Author:
Hongming ZHANG
;
Qing HUANG
;
Yang LUO
;
Chunyan YAO
;
Guangjie WANG
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae subsp pneumoniae;
Bacteriophage;
Lytic bacteriophage;
Optimal multiplicity of infection
- From:
Chinese Journal of Nosocomiology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To characterize bacteriophage isolated from sewage with indicator of Klebsiella pneumoniae subsp pneumoniae.METHODS Bacteriophage was isolated from sewage by double-layer agar plate method,identified lytic or lysogenic capacity by induced methods of ultraviolet ray and mitomycin C,performed one-step growth experiments,decided the optimal multiplicity of infection,and its structure was observed with electron microscope.RESULTS The study found a strain of bacteriophage against the K.pneumoniae subsp pneumoniae.The phage had a head of 180 nm in diameter and a tail of 210 nm in length.The plaque was transparent and 4-7 mm in diameter.the optimal multiplicity of infection was 4,latent period was 35 min and burst period was 40 min,the average burst size was about 94 PFU/cell.CONCLUSIONS The isolated bacteriophage belongs to Stylovinidae,and it is a lytic bacteriophage with narrow host spectrum.Moreover,it is only sensitive to a part of K.pneumoniae subsp pneumoniae and a few of Escherichia coli strains.
