Typing of Meticillin-resistant Staphylococcus aureus by Randomly Amplified Polymorphic DNA
- VernacularTitle:耐甲氧西林金黄色葡萄球菌随机引物DNA扩增多态性分型研究
- Author:
Changshun YANG
;
Wenen LIU
;
Xian LI
;
Mingxiang ZOU
- Publication Type:Journal Article
- Keywords:
MRSA;
RAPD;
Nosocomial infection
- From:
Chinese Journal of Nosocomiology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To acquire the information about the gene type and epidemic condition of the hospital to provide scientific proof for monitoring and controlling nosocomial infection.METHODS Meticillin-resistant Staphylococcus aureus(MRSA) was identified by its resistance to cefoxitin of disk diffusion and mecA PCR,randomly amplified polymorphic DNA(RAPD) was carried out with the optimization condition.RESULTS The rate of MRSA infection was 72.15% and the main gene type was A in the hospital.CONCLUSIONS The nosocomial infection may exist in the hospital and the hospital must take effective measure to decline nosocomial infection of the MRSA;RAPD is suitable for molecular epidemiology with high powerful discrimination,simplicity and rapidness.
