Effect of arsenic trioxide on anti-dsDNA antibody and lymphocyte subsets proliferation in splenic cells of lupus-prone MRL/lpr mice
- VernacularTitle:三氧化二砷对MRL/lpr狼疮鼠抗ds-DNA抗体和脾淋巴细胞亚群增殖的影响
- Author:
Xiaobing WANG
;
Shenghui ZHANG
;
Xianfei ZOU
;
Yan ZHOU
;
Xiaochun ZHU
;
Chaosheng CHEN
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
systemic Lupus erythematosus;
MRL/lpr mice;
Lymphocyte subsets;
NK cell
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of arsenic trioxide(ATO) on anti-dsDNA antibody and lymphocyte subsets proliferation in splenic cells of MRL/lpr mice.Methods MRL/lpr mice were divided into three groups: ATO group(0.4 mg/kg?d,ip),sodium chloride(NS) group(0.25 mL,ip),and cyclophosphamide(CTX) group(50 mg/kg?qw,ip).The control group consisted of 12 syngeneic normal C57/BL mice,which were sub-divided into ATO group(0.4 mg/kg?d,ip) and NS group(0.25 mL,ip).After two-month treatment,all mice were killed and their bodies and spleens were weighed.Anti-dsDNA antibody in serum were detected by ELISA.The percentage of different cell subsets was checked through flow cytometry.Results(1)The spleen index、serumlevel of anti-dsDNA antibody and the percentages of CD19+、CD3+、CD3+CD4+ lymphocytes in ATO group were much lower than those in NS group of MRL/lpr mice(P0.05). Conclusion Arsenic trioxide can inhibit the activation of T and B lymphocytes,increase the percentage of NK cell and reduce the serum level of auto-antibody.And it was of no effect in the normal mice.
