Trastuzumab decreases the proliferation of endothelium induced by TCM from MDA-MB-231
- VernacularTitle:曲妥珠单抗降低乳腺癌细胞条件培养液促血管内皮细胞增殖
- Author:
Ailing LI
;
Quancai CUI
;
Hongwei LI
;
Jie SHI
;
Qiang SUN
;
Chunling ZHANG
;
Ruijuan XIU
- Publication Type:Journal Article
- Keywords:
trastuzumab;
HER-2;
conditioned medium;
endothelium
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of trastuzumab,a special blocker of epidermal growth factor receptor-2(HER-2) on proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs) exposed to tumor conditioned medium(TCM).Methods HUVECs were cultured and exposed to TCM and trastuzumab was used as an intervention approach.Their proliferation,apoptosis and cell cycle distribution were measured by MTT and flow cytometery(FCM).Intra-tumor microvessel density(MVD) and HER-2 expression were tested by immunohistochemistry(IHC).Results Following the stimulation of MDA-CM,HUVECs showed higher proliferating and anti-apoptotic ability(P
