Pseudolaric acid B inhibits growth of human gastric carcinoma cells in vitro
- VernacularTitle:土槿乙酸抑制体外人胃癌细胞的生长
- Author:
Aiguo MENG
;
Jun SHI
;
Chunyan LIU
;
Hua YIN
;
Lingling JIANG
- Publication Type:Journal Article
- Keywords:
PPAR?;
gastric carcinoma;
cell cycle;
apoptosis;
Pseudolaric acid B
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pseudolaric acid B(PLAB) on growth of human gastric carcinoma cells in vitro.Methods The expression of PPAR? was detected by RT-PCR;the effect of PLAB on cell growth was tested by MTT;Hoechst33342/PI and DNA gel electrolysis were employed to examine apoptosis;cell cycle was checked by flow cytometry.Results When treated with 0.1～10 ?mmol/L PLAB for 72,the proliferation of MGC803 cells was significantly inhibited.The proportion of MGC803 cells at G2 phase was significantly increased when treated with 10 ?mmol/L PLAB after 48 h,and showed an apparent G2 phase arrest.After treatement with PLAB for 72,typical apoptotic changes were observed.The expression of PPAR? was at a low level in MGC803 cells and up-regulated when treated with 10 ?mmol/L PLAB for 48 h(P
