Hypoxia dependent HIF-1? upregulates the expression of HPA in human ovarian cancer cell line SKOV3
- VernacularTitle:低氧通过HIF-1?上调卵巢癌细胞系SKOV3中HPA的表达
- Author:
Caixia YUAN
;
Shuang GU
;
Shuhong ZHANG
;
Xiangning ZHANG
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
hypoxia inducible factor-1?;
heparanase;
rapamycin
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hypoxia inducible factor-1?(HIF-1?) and heparanase(HPA),and their relationship during hypoxia in human ovarian cancer cell line SKOV3.Methods SKOV3 cells were incubated in different groups,immucytochemistry was applied to qualitatively assay HPA protein,Western-blot was used to detect the protein expression of HIF-1? and HPA,and mRNA expression was determined by RT-PCR.Results The positive expression of HPA localized in cytoplasma.Western-blot analysis revealed that the protein expression of HIF-1? was significantly increased(P
