Role of TRAF2 in AP-1 signaling pathway of human B cells
- VernacularTitle:TRAF2在人B淋巴细胞AP-1信号传导系统中的作用
- Author:
Wen ZHANG
;
Xuan ZHANG
- Publication Type:Journal Article
- Keywords:
human ramos B cells;
TRAF2;
AP-1 signaling pathway
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2(YFP-WT-TRAF2) or YFP fusion dominant-negative TRAF2(DN-TRAF2),or transfected with shRNA-TRAF2 and control shRNA plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by antibiotics G418.Activation of AP-1 pathway,including phosphorylation of ERK,JNK and P38,as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA.Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38,and further induced nuclear translocation of C-FOS.Moreover,both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38,and nuclear translocation of C-FOS.Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway,and plays an important role in AP-1 activation.
