Effects of interventional administration of L-Arginine or nitroglycerin on acute local cerebral ischemia in rats
- VernacularTitle:介入给予左旋精氨酸及硝酸甘油对大鼠急性局部脑缺血模型的作用
- Author:
Xiangyu PIAO
;
Changkai SUN
;
Hui MA
;
Peiyang ZHOU
- Publication Type:Journal Article
- Keywords:
L-Arginine;
nitroglycerine;
ischemic stroke;
OX-42
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of treating acute ischemic stroke in rats with interventional administration of nitric oxide precurcer L-Arginine or nitric oxide donor nitroglycerin.Methods The right middle cerebral arteries of rats were occluded by insertion of a suture to duplicate ischemia-reperfusion models.Forty-two male SD rats were randomly divided into four groups: MCAO group(n=12);sham operation group(n=6);NG group(n=12) and L-ARG group(n=12),intracarotid arteries administrated respectively by NS、NS、NG and ARG.Each of the four groups were subdivided into 2 groups according to the reperfusion time(3 h and 24 h),measurement of Longa scores,NO2-/NO3-in serum,HE staining and immunohistochemical(SABC)method were utilized to assess the changes of ischemic brain tissues in different groups.Results OX-42 positive cells of cortex and CA3 area of hippocampal: OX-42 positive cells were found,their features identified at 3 h after reperfusion.24 h the response of microglias was obvious,the number of the cells increased(P
