Improved Preparation of Pure Alive Eggs of Schistosoma japonicum
- VernacularTitle:日本血吸虫纯净活虫卵制备方法的改进
- Author:
Yanhai WANG
;
Hongjuan PENG
;
Jinbao GU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Egg preparation;
In vitro culture
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To prepare a large amount of pure alive Schistosoma japonicum eggs, rabbit was infected with 2000 cercariae and its liver was taken aseptically 38-45 days after infection and homogenized. The homogenate was screened through different sieves(60, 120, 200, 300, 360 meshes per inch respectively), and washed with 1.2% NaCl. The eggs and leftover were then digested with 0.25% trypsin for 2 hours, sieved over 360 meshes per inch and washed with RPMI 1640 medium. The collected eggs reached to (95.1?6.4)% of live eggs, with a high efficiency.
