Experimental study on biological character of rabbit lingual keratinocyees

VernacularTitle:兔舌黏膜细胞的体外培养及生物学特性研究
Author: Zhengyu GU ; Jun ZHOU ; Lei CUI ; Shuo YIN
Publication Type:Journal Article
Keywords: lingual keratinoeytes; cell culture; cornea; tissue engineering
From: Chinese Journal of Primary Medicine and Pharmacy 2009;16(3):455-457,后插四
CountryChina
Language:Chinese
Abstract: Objective To explore the cultural method of lingual keratinocytes and make preparations for further investigation in using lingual keratinocytes as a new choice of seed cells for the reconstruction of tissue engineered corneal epithelium.Methods Keratinocytes were enzymatically dissociated from lingual tissue specimens using 0.25%disepase and 0.05%trypsin.The biological characteristics of the cells were observed through phase microscope.Meanwhile.1ingual kemtinoeytes were immtmohistochemically labeled with broad spectrum keratin antibody (AE1/AF3).Results The cells revealed the form of "road metal" and could be passed for 3 or 4 generations.Immunohistochemistry indicated that lingual keratinocytes were positive for AE1/AE3 staining.Condusion Lingual kemtinocytes of rabbits can be cultured with KSFM in vitm and magnitude quantity can be attained,laying a favorable foundation for lingual keratinocytes as a new choice of seed cells for corneal epithelium reconstruction with tissue engineering.