High expression of the L-Methionine ?-lyase gene in E. coli
- VernacularTitle:蛋氨酸裂解酶基因在大肠杆菌中的高效表达
- Author:
Baikun MA
;
Hongbing WANG
- Publication Type:Journal Article
- Keywords:
L-Methionine ?-lyase;
Gene cloning;
Reverse transcription;
in vitro expression;
Purification
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify and highly express the L-Methionine ?-lyase gene from Trichomonas vaginalis(TVMGL).Methods: The TVMGL gene was cloned into pGEX 4T-2,the recombinant protein expressed in E.coli DH5?and confirmed by SDS-PAGE.Results: Denaturing SDS-PAGE analysis confirmed the predicted size of the fusion protein(GST-TVMGL,68 000) and showed high purity after subsequent affinity chromatography on Glutathione sepharose 4B.The target proteins amounted to 34% of the total bacteria proteins.Conclusion: The L-Methionine ?-lyase gene was highly expressed in E.coli.
