Screening Anti-MRSA Traditional Chinese Materia Medica by Targeting on Soluble Penicillin Binding Protein 2a
- VernacularTitle:以可溶性PBP2a为靶点筛选拮抗MRSA中药的实验研究
- Author:
Yan DONG
;
Bin LI
;
Ping LUO
;
Yupeng LONG
;
Jun LI
;
Xianyuan WANG
;
Hong ZHOU
- Publication Type:Journal Article
- Keywords:
Meticillin-resistant Staphylococcus aureus(MRSA);
Penicillin binding protein 2a;
Gene expression;
Biosensor;
Traditional Chinese materia medica
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To screen the anti-meticillin-resistant Staphylococcus aureus(MRSA)traditional Chinese materia medica(TCMM)by biosensor technique,targeted on the soluble penicillin binding protein 2a(PBP2a)of clinical MRSA.METHODS The soluble PBP2a with amino acid sequence from 25 to 668 from clinical MRSA were expressed in Escherichia coli by gene recombination technique.Then,the expressed product was identified and its biological function was analyzed.After the PBP2a was immobilized into the carboxymethyl dextran cuvette(CMD),the anti-MRSA TCMM was screened by means of biosensor.RESULTS The soluble protein PBP2a had been successfully expressed,whose relative molecular mass was 74?103.It was confirmed that the soluble PBP2a had transpeptidase activitiy and ?-lactamase activitiy.Subsequently,10 kinds of anti-MRSA TCMM were screened out by biosensor technique.Moreover,Radix Scutellariae,Rhizoma Coptidis and Spica Prunellae had greater anti-MRSA effect than others.CONCLUSIONS Anti-MRSA TCMM has been successfully screened out by biosensor technique,targeted on the soluble PBP2a of clinical MRSA.
