Genotyping of ?-Lactamases and Preliminary Discovery of Acinetobacter-derived cephalosporinases AmpC Subtype from Acinetobacter baumannii
- VernacularTitle:鲍氏不动杆菌?-内酰胺酶基因型研究及ADC型AmpC酶基因的发现
- Author:
Xiaomao QIAN
;
Zuhuang MI
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
?-Lactamases;
Gene type;
Acinetobacter-derived cephalosporinases
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the genes associated ?-lactamases in Acinetobacter baumannii(ABA)isolated and preliminary discovery of Acinetobacter-derived cephalosporinases(ADC)-subtype in Shaoxing,Zhejiang.METHODS Eighteen ?-lactamases genes were detected by PCR,and a new ADC ?-lactamases gene fragments produced by PCR were sequenced and analyzed.RESULTS The positive rates of ADC-gene,TEM-gene and OXA-23 group-gene were 85.9%,27.1% and 58.8%,respectively.The other genes were not found in all 85 isolates tested.CONCLUSIONS There are very high positive percentages of ADC,TEM and OXA-23 group genes in A.baumannii isolated clinically and that is the important mechanism of multi-resistance.The ADC gene may be a novel subtype in lactamases(GenBank accession number:EF569590).
