Detection of Quinolone Resistance Gene in Enterobacter cloacae
- VernacularTitle:阴沟肠杆菌喹诺酮耐药基因的检测
- Author:
Yan LI
;
Shuzhen XU
;
Jianrong SU
;
Zhiyuan LIU
- Publication Type:Journal Article
- Keywords:
Quinolone resistance gene;
Enterobacter cloacae;
?-Lactamases;
Drug resistance
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the appearance of quinolone resistance gene(qnr)in Enterobacter cloacae for clinical treatment.METHODS E.cloacae was identified by VITEK-AMS;detection of qnr and extended-spectrum ?-lactamases(ESBLs)resistant genes were performed by PCR.Plasmid conjugation test was used to determine the transmission of qnr.The antibotic susceptibility of E.cloacae was detected according to the CLSI guideline.RESULTS The incident rate of E.cloacae with qnr was 23.6% in 55 strains.The sensitive rate to ciprofloxacin was 46.2% in isolates with qnr,but it was 83.3% in isolates without qnr.qnr Genes of 38.2% ESBLs isolates were positive.Two strains harboring qnr genes were conjugated successfully.CONCLUSIONS ESBLs strains usually harbor qnr genes.qnr Genes can be horizontally transferred to recipient by plasmid conjugated test.
