Inhibitive Effects of Somatostatin on LPS-stimulated Rat Kupffer Cells
- VernacularTitle:生长抑素对脂多糖刺激的大鼠库普弗细胞抑制作用的观察
- Author:
Jinghong YAO
;
Yongwen HE
;
Shenghua JIE
- Publication Type:Journal Article
- Keywords:
Kupffer cells;
Endotoxin;
Somatostatin;
NO;
TNF?;
5-Lipoxygenase
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of somatostatin(SST)on expression of NO,TNF? and 5-lipoxygenase(5-LO)in primary cultured rat Kupffer cells induced by LPS.METHODS Kupffer cells isolated from Sprague-Dawley rats were cultured in the presence of LPS added alone or with SST for 12,24 and 48 h,and the production of TNF? was assessed in culture supernatants by ELISA and that of nitric oxide(NO)by nitrate reductase method.5-LO mRNA expression was assessed by semiquantitative RT-PCR.RESULTS Vehicle-stimulated Kupffer cells produced a basal amount of NO,TNF? and expressed 5-LO mRNA.Kupffer cells stimulated by LPS secreted significantly increased amounts of NO and TNF?(P0.05).CONCLUSIONS SST modulates NO,TNF? production and 5-LO mRNA expression by LPS-stlimulated Kupffer cells.
