Role of three different extracellular matrixes in the activation of rat microglia
- VernacularTitle:三种细胞外基质蛋白对大鼠小胶质细胞活化的影响
- Author:
Zheng XUE
;
Suming ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Integrins act as receptors for molecules of the extracellular matrix (ECM) and play a major role in mediating cell morphology change,metabolism,function,migration,proliferation and differentiation. It is still unclear that whether ECM is associated with regulation of microglial activation and integrin expression. The study investigated the effects of three different ECM (fibronectin, vitronectin and laminin) on the expression of rat microglia integrin. METHODS:Experiments were performed in Research Center for Clinical Neurology, Tongji Medical College of Huazhong University of Science and Technology from May 2006 to August 2007. ①Neonatal Wistar rats were bought from Experimental Animal Center of Tongji Medical College. Animal intervention met the animal ethical standard. ②Rat microglia was cultured and purified. Culture plate was coated with fibronectin, vitronectin and laminin, and non-ECM served as control. ③The influence of individual ECM on microglia integrin expression and microglia surface expression of MHC Ⅰ by flow cytometry. RESULTS:①MHC classⅠexpression on rat microglia was significantly increased by fibronectin and vitronectin(P
