Quality specification of human embryo olfactory ensheathing cells from olfactory bulb
- VernacularTitle:人类胚胎嗅球嗅鞘细胞质量标准
- Author:
Yushui REN
;
Guozhong TIAN
;
Hongmei WANG
;
Hongyun HUANG
;
Lin CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to control the quality of all parts of the process for culturing,preparing and using clinically the human embryo olfactory ensheathing cells (OECs),the conduct standard of culturing human OECs from olfactory bulb is formulated. Because the strict conduct process can reduce the human factor as much as possible and ensure the stability of the cell quality. Therefore,during the culturing process of human embryo OECs, as for the embryo sample of OECs from olfactory bulb,the standard of lab condition and cell culture must be set strictly. The key steps of conduct process must be regulated,the freezing and resuscitating process of OECs must be controlled,the test method for microbial contamination during cell culture must be proposed and the risk must be reduced,then the quality of the human OECs from olfactory bulb can be guaranteed during the clinical application.