Development and Application of New Substrate-stretching Device for Cultured Cells
- VernacularTitle:体外细胞基底拉伸加载装置的研制与应用
- Author:
Dong XU
;
Qiping HUANG
;
Chong MEI
;
Xiao CHEN
;
Junpei XIA
- Publication Type:Journal Article
- Keywords:
cell culture;
substrate-stretching device;
changes of morphology and cytoskeleton
- From:
Chinese Medical Equipment Journal
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To simulate the mechanical environment of cells in vivo and study cellular signal transduction mechanisms. Methods A device was developed which could provide high cell yield, control the time, strain magnitude, direction and frequency of stretch, and applied 10% cyclic strain to cell culture substrate with stretch frequency at 1Hz. Results After being stretched, morphology and cytoskeleton of cells were altered. The major axis of cells and the alignment of stress fibers were vertical to the orientation of cyclic stretch. Conclusion This device provides versatile options for the study on the cellular responses of mechanical loading.
