Protective effects of polysaccharides from Hyriopsis cumingii on hepatocytes injured by CCl_4
- VernacularTitle:珠蚌多糖对四氯化碳诱导肝细胞损伤的保护作用
- Author:
Lei CHEN
;
Hao WU
;
Jingqian YAO
;
Nian CHANG
- Publication Type:Journal Article
- Keywords:
polysaccharides from Hyriopsis cumingii;
L-02 hepatocytes;
CCl4;
anti-oxidative activity
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect on CCl4-injured L-02 hepatocytes of polysaccharides from Hyriopsis cumingii(HCP) and try to explain the mechanism.Methods The L-02 hepatocytes were incubated and then injured by CCl4.The levels of AST,ALT,MDA and SOD in cultural supernatant were detected by general methods.Cell viability was assayed by MTT method.Results The polysaccharides(25,250 and 1000?g?L-1)could reduce the levels of AST,ALT,MDA in cultural supernatant which increased by CCl4.It also could boost the hepatocytes viability and elevate the level of SOD which reduced by CCl4.Conclusion The results suggest that polysaccharides from Hyriopsis cumingii have direct protective activity on hepatocytes injured in vitro.It might be associated with the anti-oxidative activity of polysaccharides from Hyriopsis cumingii.
