The application of mRNA differential display in screening differential expressedgenes of peripheral blood leucocyte from Uigur and Kazak patients with type 2 diabetes mellitus
- VernacularTitle:mRNA差异显示法在维吾尔族和哈萨克族2型糖尿病患者白细胞差异基因筛选中的应用
- Author:
Linyue ZOU
;
Zhaoxia ZHANG
;
Qiong ZHANG
;
Qin WEI
;
Feiya ZHUO
- Publication Type:Journal Article
- Keywords:
Uigur;
Kazak;
type 2 diabetes mellitus;
mRNA differential display;
gene
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the differentially expressed genes in Uigur and Kazak patients with and without type 2 diabetes mellitus.Methods The differentially expressed cDNA bands were isolated by fluorescent mRNA differential display from peripheral blood leucocyte of the Uigur and Kazak patients with type 2 diabetes mellitus and the normal controls. After being cloned, all cDNA fragments were sequenced, then underwent sequence analysis, homogenous comparison,and Northern blot analysis. Results Z5、Z8、Z15 differentially expressed cDNA fragments were found.They were over-expressed in the normal controls and were lower or scarced in the Uigur and Kazak patients with type 2 diabetes mellitus. They were selected for sequencing and hybridization. Z5、Z8 showed highly homologous to cellular repressor of E1A-stimulated genes,Z15 are unknown. Conclusion Three differentially expressed genes may have a potential relation with type 2 diabetes mellitus.
