In vitro differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neurons
- VernacularTitle:大鼠骨髓间充质干细胞向多巴胺能样细胞的体外分化
- Author:
Li CHEN
;
Dongmei HE
;
Xudong JING
;
Huan ZHANG
;
Baoying FANG
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells;
differentiation;
dopamine;
rat
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neuron.Methods The mesenchymal stem cells were isolated from bone marrow. They were induced to differentiate into dopaminergic neurons by freated with bFGF, VitC and EGF at the third generation. Dopamine-associated protein and genes in the treated cells were examined by immumofluorescence and RT-PCR. Dopamine in the supernatant and endoplasm from culture system was determined by ELISA kit. Results The results showed that tyrosine hydroxylase, dopamine transporter and nerve neucleoprotein and Nestin,Nurr-1 genes were found. And the dopamine existed in the supernatant and cytoplasm from inducing culture system. Conclusion The rat bone marrow mesenchymal stem cells have the capacity of differenting into dopaminergic nurons.
