Effects of astragalosides on proliferation and cell cycle of rat glomerular mesangial cells
- VernacularTitle:黄芪皂甙对大鼠肾小球系膜细胞增殖及周期的影响
- Author:
Jinfang SUN
;
Jinju JIAO
;
Qiman SONG
;
Yuhong BAI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of astragalosides (AS) on the proliferation and cell cycle of rat glomerular mesangial cell (MC), and verify the correlation between the influence and the AS concentration. METHODS: The experiment was carried out in the Physiological Laboratory of Liaoning Medical University from December 2006 to July 2007. Rat glomerular MCs were cultured in high glucose for 4-7 passages. The experiments were randomly divided into control group and three AS groups with different concentrations (50, 100, 200 mg/L), which were treated with high-glucose liquid and AS respectively. MC proliferation was determined with MTT colorimetric method in each group at hours 48 after intervention. MC cell cycle was detected with flow cytometry. RESULTS: ①MTT results showed that, the values of A490 nm in AS groups were lower than that in the control group at hours 48 (P
