Influence of interferon-gamma in combination with methylprednisolone on the proliferation of human embryonic lung fibroblast
- VernacularTitle:干扰素?与甲强龙联合应用对人胚肺成纤维细胞增殖的影响
- Author:
Xuemei LI
;
Lidong ZHAI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The pathogenesis of interstitial lung disease is still uncertain. This study observed the effect of interferon-gamma (IFN-?) in combination with methylprednisolone (M-pred) on the proliferation of human embryonic lung fibroblast (HELF) to identify whether it can be used as an effective treatment of interstitial lung disease in clinic. METHODS: The experiment was performed at the Research Laboratory of Cytobiology, Hebei Medical University from July 2005 to May 2006. ①The eighth passage HELFs were cultured in vitro. According to the common dose range for human of IFN-? and M-pred, cells were divided into control group, M-pred (15 mg/L) group, IFN-? (250, 500, 750, 1 000, 1 250 U/mL) groups and combination group (IFN-? and M-pred). ②HELFs were incubated with IFN-? alone or combined with M-pred for 48 hours before they were harvested. ③MTT assay was used to measure the inhibition rate of M-pred combined with different concentrations of IFN-? on HELF. Flow cytometry was used to observe cell cycle distribution and the proliferation index of HELF. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemistry. RESULTS: ①Both alone and combination usage of IFN-? and M-pred could inhibit HELF proliferation (P 0.85). ② IFN-? mainly acted on G1 and G2M periods, while M-pred on S period. The proliferation index decreased in combination group (P
