Effect of bone marrow stromal cells transfeted by human sFlt-1 gene on growth of K562 cells
- VernacularTitle:人sFlt-1基因转染骨髓基质细胞对K562细胞生长的影响
- Author:
Qian WANG
;
Yanqiu SONG
;
Yan TAN
;
Xiumei DUAN
;
Lihua LIU
- Publication Type:Journal Article
- Keywords:
receptor,growth factor;
endothelium,vascular;
transfection;
bone marrow stromal cells;
K562 cells
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To get the protein expression of sFlt-1 by transfection and to investigate the effects of sFlt-1 gene transfection on the growth of K562 cells.Methods The recombinant plasmid pcDNA3-sFlt-1D4 was constructed.The recombinant plasmid pcDNA3-sFlt-1D4 was transfected into the bone marrow stromal cells by Lipofectamine 2000,which was identified by RT-PCR,ELISA and MTT.Results The transfection efficiency identified by flow cytometry was 9.27%.The protein expression of sFlt-1D4 was found in the culture supernatant 24 h,48 h and 4 weeks after transfetion by ELISA and the expression concentrations were(0.104?0.078),(0.158?0.022) and(0.171?0.069) ?g?L-1,respectively.The content of VEGF secreted by K562 culturing with transfectant cells culture supernatant was reduced compared with control.The inhibitoy rates on the proliferation of K562 cells via MTT assay were 9.41%?4.71%,23.63%?7.50%,and 33.13%?6.93%,respectively.Conclusion The bone marrow stromal cells transfected with recombinant plasmid pcDNA3-sFlt-1D4 could secrete sFlt-1D4 and inhibit the proliferation of K562 cells.
