Protective effect of gross saponin tribulus terrestris on apoptosis of PC12 cells induced by H_2O_2
- VernacularTitle:蒺藜总皂苷对H_2O_2诱导PC12细胞凋亡的保护作用
- Author:
Enping JIANG
;
Hong LI
;
Yingshi JI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
gross saponins tribulus terrestris;
PC12 cells;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of gross saponin tribulus terrestris(GSTT) on the apoptosis of pheochromocytoma cells(PC12 cells)induced by H2O2 and its mechanisms.Methods PC12 cells were divided into control,model,high dose GSTT(GSTT1) and low dose GSTT(GSTT2) groups.Apoptosis of PC12 cells was induced by H2O2 at the concentration of 300? mmol?L-1.The cell activity was determined by MTT.The subdiploid peaks showing cell apoptosis rate and ?m were detected by flow cytometry.Proteins of Bcl-2 and Bax were detected by immunohistochemistry.Results Compared with model group,the survival rate of PC12 increased(P
