Induction of retinoic acid on PC12 cells differentiated into neuron-like cells
- VernacularTitle:维甲酸对诱导PC12细胞向神经元样细胞分化的影响
- Author:
Xiuli WANG
;
Chen LI
;
Jiamei LIU
;
Dong CHEN
- Publication Type:Journal Article
- Keywords:
PC12 cells;
retinoic acid;
differentiation;
MAP2
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the condition of pheochromocytoma cells(PC12 cells) differentiated into neuron-like cells induced by retinoic acid(RA) and to observe the lengh of neurite and max diameter and the expression of MAP2 in PC12 cells during their differentiation into neuron-like cells.Methods There were seven groups :0.1,0.3,0.5,1.0,2.0 and 5.0 mg?L-1RA groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to RA with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to RA for 72 h.Results The number of MAP2 positive cells was significantly increased in 0.3,0.5,1.0,2.0 mg?L-1RA groups compared with control group and the most significant concentration was 1.0 mg?L-1(P
