Effect of arsenic trioxide on telomerase activity and apoptosis of human colon cancer cell Line
- VernacularTitle:三氧化二砷对人大肠癌细胞端粒酶活性及细胞凋亡的影响
- Author:
Dan XU
;
Youlin YANG
;
Yanjun WANG
- Publication Type:Journal Article
- Keywords:
arsenicals;
apoptosis;
telomerase;
telomere
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Abstract:Objective To explore the effects of arsernic trioxide(As2O3) on telomerase activity and cellular apoptosis of human colon cancer cell line CCL-187.Methods CCL-187 cells were divided into control group,1.0 ?mol?L-1As2O3 group and 2.5 ?mol?L-1As2O3 group,all groups were cultivated for 1 to 6 d.The inhitory rate of cell growth was detected by MTT assay.The morphologic changes of CCL-187 cells were observed under electron microscope.The expression of hTERT at mRNA level was analyzed by RT-PCR.Telomerase activity was determined by TRAP-PCR-ELISA.Results The differentiation of cells was found in 1.0 ?mol?L-1 As2O3 group,but apoptosis in 2.5 ?mol?L-1 As2O3 group,and at the same time,the telomerase activity was reduced and the hTERT-mRNA expression was downregulate in 1.0 ?mol?L-1 As2O3 group and 2.5 ?mol?L-1 As2O3 group in a time-dependent manner.There were significant differences compated with control group(P
