Expression of imprinted gene PEG10 in human gastric adenocarcinoma tissues and significance
- VernacularTitle:印迹基因PEG10在胃腺癌组织中的表达及意义
- Author:
Kai WANG
;
Yunfeng PIAO
;
Dayong DING
;
Ye FENG
- Publication Type:Journal Article
- Keywords:
genomic imprinting;
PEG10;
gastric neoplasm;
reverse transcriptase polymerase chain reaction
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of a novel imprinted gene,PEG10,in human gastric adenocarcinoma,and the effect of PEG10 on cell growth and proliferation of gastric cancer cells.Methods The PEG10 mRNA expressions in 40 human gastric adenocarcinoma,the corresponding adjacent normal tissues and 6 nomal gastric tissues were detected by RT-PCR.The expression vectors of PEG10 were constructed and transfected into gastric cancer cell line MKN45 which had no endogenetic PEG10 expression.Cell growth ability was measured by MTT assay.Results High PEG10 mRNA expression level was detected in 9 of 20(45.0%) human gastric adenocarcinoma which was significantly higher than those of the matched normal tissues(10.0%)(P
