Determination of CD4~+CD25~+ regulatory T cells from peripheral blood in patients with multiple organ dysfunction syndrome and clinical significance
- VernacularTitle:多器官功能障碍综合征患者外周血CD4~+CD25~+调节性T细胞的检测及临床意义
- Author:
Xiuxian ZANG
;
Junqi NIU
;
Yanfang JIANG
- Publication Type:Journal Article
- Keywords:
multiple organ failure,CD4~+CD25~+CD127-Treg;
infect;
immune regulate
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the change of CD4+ CD25+ regulatory T cells(Treg) from peripheral blood in patiens with multiple organ dysfunction syndrome(MODS) before and after treatment and its significance.Methods The frequencies of CD4+ CD25+ CD127-Treg were detected in 10 patients with MODS respectively before treatment and 1 week after treatment and 10 healthy donors by flow cytometry labeled with specific fluorescent antibodies,such as anti-CD4(PE-CY5),anti-CD25(FITC) and anti-CD127(PE).Results The frequency of CD4+ CD25+ CD127-Treg in patients after treatment(2.11%?0.33%) was significantly lower than before treatment(7.44%?1.59%)(P
